Learn How to Properly Use Growth Hormone for Injection, Dosing & Mixing

Use Real Human Growth Hormone for Your HGH Treatments as Prescribed by your Doctor

Genuine Growth Hormone Real HGH comes in the form of a crystalized white lyophilized powder or in a pen cartridge device marked as rDNA Somatropin. Be sure when you buy injectable HGH you are getting true HGH.

The Human Growth Hormone Shot. The only proper way to administer true HGH is by subcutaneous injection. Please keep in mind that HGH is a fragile hormonal substance and needs to be protected from both heat and light. while some forms of somatropin can be stored at room temperature, most must be refrigerated.

How to store your HGH Injections. Somatropin HGH should be stored in a cool refrigerator at 36 – 46 degrees Fahrenheit, or 2-6 degrees Centigrade before and after reconstitution. There are some growth hormone injection products on the market that don’t require refrigeration like certain Saizen injections from Serono, but you must read the label and your doctor’s HGH prescription instructions carefully. Almost all brands of injectable somatropin (HGH) need to be shipped with ice packs and stored in a refrigerated location in order not to lose their potency and bio-activity. While some approved high quality somatropin brands can tolerate normal room temperature during storage and shipping that only applies before the HGH powder is mixed and reconstituted. Please keep in mind, all injectable HGH needs to be refrigerated after being reconstituted by mixing the HGH lyophilized powder with the bacteriostatic water.

Self-Administration - Giving Yourself the HGH Shot

Human Growth Hormone (Somatropin) Shots may be self-administered. Read this document carefully and fully prior to first administration. The following information has been prepared for safe and effective self-administration of your injectable therapies. HGH Injections are now available in Injectable HGH pens that are either disposable or have refillable hormone drug cartridges. These HGH pens make measuring your HGH shot dose and injecting your hormone medication fast, simple and pain-free. You can contact us here at Optimal Health Medical for assistance – 1-888-763-4221

HGH Drug Vial & Syringe. Many of the brand name manufacturers of approved injectable HGH offer pen devices for self-injection of your daily hormone shots. These GH drug pen devices come pre-measured in single or multi-dose cartridges or allow you to measure the precise dose of Growth Hormone for your injections. These innovation in HGH treatment delivery methods make taking your meds easier and quicker. Some patients still use a vial of lyophilized GH powder and mix it with bacteriostatic water to create a solution for their GH shot and they look for guidance on how to properly inject HGH and at the right dose as prescribed by their doctor. If you are using a traditional Vial and Syringe kit, make sure to follow the injection protocol for HGH carefully per below. This will help you avoid any unwanted Side Efffects of HGH.

Obtain Real HGH from a Licensed Medical Specialist

Preparing for self-administration of HGH injections:

1.) Wash hands thoroughly with soap and warm water.
2.) Select a well-lit, clean hard surface preferably with a mirror (e.g. Bathroom counter top).
3.)Ensure you have all necessary supplies and that all packaging and wrappings are intact and there are no signs of tampering. Double check the expiration dates on all medications. Note: With Testosterone (Intramuscular injections) You will use (2) two needles: (1) one needle for drawing the medication, referred to in these instructions as the "drawing needle" and (1) one needle for administration of the solution, "administration needle".

Drawing HGH solution into syringe:

  • Use alcohol wipes to clean the tops of the vials – move in one direction and use one wipe per vial.
  • Remove syringe with needle from packaging and fill syringe with an equal amount of air as the intended dose by pulling plunger back to the desired mark (i.e. If you are drawing up 2 ml’s of solution you would pull plunger to the “2” on the syringe).
  • Insert the needle straight through the rubber stopper, into the vial. If you touch the stopper with your finger clean it with a fresh alcohol wipe prior to withdrawing medication.
  • Turn the medication vial upside down keeping the needle tip inside the liquid.
  • Inject the air into the medication vial. This helps to maintain a balance of internal/external pressure when using glass vials;this also makes future medication withdrawal easier.
  • Hold the vial and syringe in one hand and slowly draw up that amount of liquid into syringe. Diagram 1 shows technique on holding vial.
  • While holding the vial upside down and maintaining drawing needle inside the vial, gently tap the side of the syringe until air bubbles rise to the top of the barrel of the syringe. Gently push the plunger to eject ONLY THE AIR BUBBLES through the needle.
  • Remove needle and syringe from vial.
  • Recap needle by placing the plastic needle cap on countertop and safely guiding the needle into the cap. This reduces the possibility of accidentally sticking yourself.
  • For Testosterone and/or IM injections remove the drawing needle and discard it in an OSHA approved Sharps container. Federal Regulations require needles and sharps objects to be disposed of in a proper bio-medical waste container. If you are missing or need sharps containers please call us immediately. Make sure to always properly dispose of all used needles.

Injecting of HGH solution:

Keep syringes and materials clean and do not let anything touch the tip of syringe or needle. If you accidentally touch the tip or accidentally contaminate it in any way you must discard and start all over. This eliminates the possibility of infection.

  1. For Testosterone or IM injections Remove fresh administration needle from packaging and inspect for any defects. Do not remove plastic cap until ready for injection.
  2. Firmly twist administration needle on to syringe and maintain in front of you on counter top until you are ready for administration.
  3. For SUBQ injections the small insulin syringe is preloaded with needle which cannot be removed and will be used for administration.
  4. Use an alcohol wipe to clean skin at the injection site;let it air dry. Throw away the wipe. NOTE: Do not use any areas in which you feel lumps, bumps, firm knots or pain. Do not use any area in which the skin is discolored, depressed, scabbed, or is broken open. If you are in doubt call your representative.
  5. Remove the needle cap from the needle and gently pinch the skin together around the site (to lift it up a bit) See Diagram 2 . The best areas for injection are away from joints, nerves, bones, and other important body. Refer to last page for injection site diagram. The preferred areas for Intramuscular (IM) injections, such as Testosterone, are the gluteus maximus (buttocks) and the quadriceps muscles. The preferred area for Subcutaneous (SUBQ) injections, such as Growth Hormone and HCG is around the navel, stomach area.
  6. With a swift motion stick the needle into the skin at a 90 angle for intramuscular (IM) injections and at a 45 angle for subcutaneous (SUBQ) injections. *STEP 7 is for INTRAMUSCULAR (IM) INJECTIONS ONLY! SUBQ injections Go to Step 8.
  7. (IM) While needle is in the muscle pull back on the plunger slightly and look for blood inside syringe. *Rarely will there be blood but if you experience this – it just means you are near or hit a blood vessel. Immediately pull needle and syringe out and gently massage area with gauze or clean tissue. Discard the needle and Go back to Step 2 in this section and repeat after selecting another injection site.
  8. Inject the medication by using a slow steady push on the plunger until the syringe is completely empty. Hold a cotton swab or gauze near the injection site.
  9. Swiftly and safely remove the needle from the skin and gently massage the injection site with a dry cotton ball or gauze.
  10. Immediately discard the syringe and the needle in an OSHA sharps container.
  11. Maintain all supplies and medication in a safe place and away from the reach of children and others. Ensure proper storage of medical supplies and refrigerated medicines.
  12. Once you have filled the OSHA approved sharps container to the “recommended fill line” you can call your local waste management and follow local guidelines for proper disposal.

Information listed in this document is provided for informational purposes and is not meant to substitute for the advice provided by your own physician or other medical professional. You should not use the information contained herein for diagnosing or treatment of a health problem or disease, or prescribing any medication. If you have or suspect that you have a medical problem, promptly contact your health care provider or your representative.


Your vial of Somatropin hGH (either OMNITROPE, NUTROPIN, SAIZEN, SEROSTIM, CRYOTROPIN, GENOTROPIN, HUMATROPE, JINOTROPIN, NORDITROPIN, PROTOPHIN etc..) is in the form of a lyophilized powder and comes with a small bottle of bacteriostatic water for mixing. The amount of the powder should be indicated on the vial, it will stated Units (IU’s) or Milligrams (mg). If in milligrams the conversion is precisely 1mg=2.7IU but they state in general taht 1mg = 3IU’s so 4IU’s equal 1.33mg. As a physician I use precise measurements but most people use the 1mg = 3IU’s since that is the standard commonly referenced by rDNA somatropin manufacturers.

Mixing the HGH powder. You will add the bacteriostatic water (or sterile water) to the lyophilized powder {some patients and physicians with experience of use over 20 years with this product use liquid vitamin B12 to reconstitute} and thus prepare for injection. What you choose to reconstitute hGH with depends on how rapidly you use the GH. Bacteriostatic water is basically sterile water with 0.9% Benzyl Alcohol added, and this Alcohol keeps anything from growing in the water, thus making it safe for injection for the longest amount of time, about three to three 1/2 weeks. If the amount of GH in our vial is enough to last for a few weeks at our desired daily dosage then bacteriostatic water is the way to go and diluent of choice. Many users {for bodybuilding use about 2-5 IU’s a day} and remember most vials will be used up long before any bacteria begins to grow in the reconstituted hGH. So it’s really a personal preference which diluent you use.


1.) Take some alcohol and swab the stopper {top of the vial} of your hGH vial and the dilutant water.
2.) Take a 3cc syringe with a 23 or 25 gauge needle (1″ or 1.5″) and draw up the amount of your preferred dilutant. The amount isn’t critical, other than making sure you know exactly how much you have used. The rule of thumb is choose an amount that will make measuring the final product easy.
Example: 1ml (cc) per 10IU vial of hGH means each 10 mark on U100 syringe equals 1 IU of HGH
2ml (cc) added to a 10 IU vial of HGH means each 20 mark on a U100 syringe equal 1 IU of HGH
3ml (cc) added to a 10 IU vial of HGH means the 30 mark on U100 syringe would equal 1 IU of HGH
3.) Then you take the syringe {already having the dilutant} and push it into the vial containing the lyophilized powder, you may want to angle it so that the needle touches the side of the vial, and avoiding injecting the dilutant directly onto the lyophilized powder, you want to make it run slowly down the side of the vial (don’t force it all at once or don’t rush it in).
4.) After all of the dilutant has been added to the vial of hGH, gently swirl (but do NOT agitate or violently shake the vial) until the lyophilized powder has dissolved and you are left with a clear liquid. The hGH is now ready for use. Store your reconstituted hGH in the refrigerator and if you used bacteriostatic water to reconstitute, it will be good for about three weeks. If you used sterile water, then it will only be good for about 4 -5 days


1.) Always look at your HGH vial. How much actual hGH you are dealing with (read the vial – is it 4IU or 10IU?)

2.) Measure your bacteriostatic water. How much water (dilutant) are you using to add to the HGH powder?

3.) Make sure you will inject the right amount of HGH Solution. Divide the amount of water in units by the amount of HGH in units.

4.) Double check your math and measurements on the HGH syringe. This result will equal the measurement on your U100 Insulin syringe per unit of HGH.

5.) Swab your injection area with an alcohol swab. Make sure you inject subcutaneously into the fatty layer, NOT into the muscle.

You can get real Injectable Growth Hormone legally and safely with your doctor’s prescription for Human Growth Hormone Replacement Therapy. Buy only FDA-approved somatropin from Pfizer (Genotropin), Novo Nordisk (Norditropin), Merck EMD Serono (Saizen), Lilly (Humatrope) or Genentech (Nutropin).